The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 06, 2022
Filed:
Jan. 31, 2019
Applicant:
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Inventors:
Arkadiusz Bibillo, Cupertino, CA (US);
Randall W. Davis, Pleasanton, CA (US);
Assignee:
Roche Sequencing Solutions, inc., Pleasanton, CA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6855 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6848 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/6855 (2013.01); C12Y 207/07007 (2013.01); C12Y 301/11002 (2013.01); C12Y 301/11003 (2013.01); C12Y 301/11005 (2013.01); C12Y 301/11006 (2013.01); C12Y 301/15 (2013.01); C12Y 301/21 (2013.01); C12Y 301/22001 (2013.01); C12Y 301/25001 (2013.01);
Abstract
Sequencing adaptors and methods are provided for preparation of polynucleotides for sequencing. The sequencing adaptors contain a portion of a recognition sequence for a methyl-dependent endonuclease. Unwanted adaptor dimers that form during ligation of adaptors to target polynucleotides produce a complete restriction sequence and are cleaved by the endonuclease, followed by exonuclease digestion, thereby removing the dimers.