The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 06, 2022
Filed:
Mar. 18, 2020
Applicant:
Qiagen Gmbh, Hilden, DE;
Inventors:
Alexander Azzawi, Solingen, DE;
Ralf Peist, Hilden, DE;
Assignee:
QIAGEN GmbH, Hilden, DE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/686 (2018.01); C12N 9/22 (2006.01); C12N 9/12 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C12N 9/1241 (2013.01); C12N 9/22 (2013.01); C12Y 207/00 (2013.01); C12Y 301/00 (2013.01);
Abstract
The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.