The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 06, 2022

Filed:

Feb. 26, 2018
Applicant:

Georg-august-universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin, Göttingen, DE;

Inventors:

Elisabeth Zeisberg, Göttingen, DE;

Xingbo Xu, Göttingen, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12N 15/113 (2010.01); C12N 9/02 (2006.01); C12N 9/22 (2006.01); C12N 15/67 (2006.01);
U.S. Cl.
CPC ...
C12N 15/102 (2013.01); C12N 9/0071 (2013.01); C12N 9/22 (2013.01); C12N 15/113 (2013.01); C12N 15/67 (2013.01); C12N 2310/20 (2017.05);
Abstract

A method for re-expression of hypermethylated RASAL1, hypermethylated LRFN2, and hypermethylated KLOTHO based on an inactivated CRISPR-based system and a DNA dioxygenase as well as a gRNA guiding the construct to the RASAL1, LRFN2, and KLOTHO gene for demethylation of hypermethylated RASAL1, hypermethylated LRFN2, and hypermethylated KLOTHO, in particular, hypermethylated RASAL1, LRFN2, and KLOTHO promoter, thus, allowing re-expression of RASAL1, LRFN2, and KLOTHO for the treatment of fibrosis, cancer or neuronal disorders in a subject is provided. A kit of parts for allowing re-expression of hypermethylated RASAL1, hypermethylated LRFN2, and hypermethylated KLOTHO in a subject, a vector or vector system, and nucleic acid constructs are also provided.


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