The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 29, 2022
Filed:
Apr. 29, 2015
Applicant:
Novartis Ag, Basel, CH;
Inventors:
Assignee:
Novartis AG, Basel, CH;
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 15/63 (2006.01); C12N 5/00 (2006.01); C12P 21/00 (2006.01); C12N 15/90 (2006.01); C12N 9/64 (2006.01); G01N 15/14 (2006.01); A01K 67/00 (2006.01); G01N 15/00 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C12N 5/00 (2013.01); C12N 9/6424 (2013.01); C12N 15/907 (2013.01); C12Y 304/21109 (2013.01); G01N 15/14 (2013.01); C12N 2510/02 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/1006 (2013.01);
Abstract
The present application pertains inter alia to an isolated vertebrate cell suitable for recombinant expression of a polypeptide of interest, wherein the vertebrate cell is altered to impair the function of the endogenous protease matriptase and wherein the cell comprises at least one heterologous polynucleotide encoding a polypeptide of interest and wherein the polypeptide of interest is secreted by the cell. It was found that using respective vertebrate cells for producing a recombinant polypeptide of interest significantly reduces clipping of the polypeptide of interest that is secreted into the cell culture medium. Also provided are improved production and screening methods.