The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 29, 2022

Filed:

Nov. 09, 2021
Applicant:

Inscripta, Inc., Boulder, CO (US);

Inventors:

Andrew Garst, Boulder, CO (US);

Tian Tian, Boulder, CO (US);

Daniel Held, Boulder, CO (US);

Assignee:

Inscripta, Inc., Boulder, CO (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/902 (2013.01); C12N 15/905 (2013.01); C12N 15/907 (2013.01); C07K 2319/00 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01);
Abstract

The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or 'RGN')/single-strand binding protein ('SSB') fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.


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