The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 22, 2022
Filed:
Nov. 08, 2016
Applicant:
Kyoto University, Kyoto, JP;
Inventors:
Hirofumi Suemori, Kyoto, JP;
Takamichi Miyazaki, Kyoto, JP;
Assignee:
Kyoto University, Kyoto, JP;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/074 (2010.01); C12N 5/00 (2006.01); C12N 5/0735 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0696 (2013.01); C12N 5/00 (2013.01); C12N 5/0606 (2013.01); C12N 2500/30 (2013.01);
Abstract
Provided is a cell culture method comprising the step of culturing cells using a medium containing a laminin fragment having integrin binding activity, the method not comprising the step of coating a culture vessel with a laminin or a laminin fragment before seeding the cells in the culture vessel. The cell culture method of the present invention uses a smaller amount of a laminin fragment and still achieves a comparable culture efficiency as compared with the conventional cell culture method that uses a culture vessel precoated with a laminin or a laminin fragment.