The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 15, 2022

Filed:

Jun. 28, 2018
Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventors:

David P. Stumbo, Pleasanton, CA (US);

George Carman, Livermore, CA (US);

Steve Hobbs, Pleasanton, CA (US);

Anthony J. Makarewicz, Jr., Livermore, CA (US);

Dmitri Simonian, Mountain View, CA (US);

David Glade, San Ramon, CA (US);

Joshua Oen, Fremont, CA (US);

Denis Pristinski, Pleasanton, CA (US);

John Dzenitis, Danville, CA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 15/14 (2006.01); B01F 3/08 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/686 (2018.01); B01L 3/00 (2006.01); C12Q 1/6851 (2018.01); B01F 23/41 (2022.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); B01F 23/41 (2022.01); B01L 3/502784 (2013.01); C12Q 1/6846 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/6851 (2013.01); G01N 15/1429 (2013.01); G01N 15/1434 (2013.01); G01N 15/1459 (2013.01); B01L 3/502776 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/0654 (2013.01); G01N 2015/1481 (2013.01);
Abstract

Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.


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