The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 08, 2022

Filed:

May. 19, 2016
Applicant:

Kws Saat SE & Co. Kgaa, Einbeck, DE;

Inventors:

Hinrich Harling, Bovenden, DE;

Susana Martin-Ortigosa, Einbeck, DE;

Markus Niessen, Hannover, DE;

Corinna Streitner, Melle, DE;

Nadine Schumann, Einbeck, DE;

Erik Jongedijk, Lokeren, BE;

Assignee:

KWS SAAT SE & CO. KGAA, Einbeck, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01); C12N 15/113 (2010.01); A01H 5/04 (2018.01); A01H 5/12 (2018.01);
U.S. Cl.
CPC ...
C12N 15/8213 (2013.01); A01H 5/04 (2013.01); A01H 5/12 (2013.01); C12N 15/113 (2013.01); C12N 15/8201 (2013.01); C12N 15/8203 (2013.01); C12N 15/8205 (2013.01); C12N 15/8207 (2013.01); C12N 2310/20 (2017.05);
Abstract

The present invention relates to methods and hybrids for the targeted modification of a nucleic acid-target region in a plant target structure using CRISPR/Cas systems. The invention specifically relates to methods and hybrids for directly obtaining a plant or plant material which comprises an editing of a nucleic acid introduced in a targeted manner into a meristematic cell. The hybrids can be introduced in a transient and/or stable manner. The invention also relates to novel plant-optimized introduction strategies. The invention further relates to a method for carrying out an in vitro screening assay in order to first check the suitable gRNA candidates in vitro with respect to their efficiency.


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