The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 18, 2022
Filed:
Sep. 04, 2014
Kyoto University, Kyoto, JP;
Osaka University, Suita, JP;
Eisai R&d Management Co., Ltd., Tokyo, JP;
Jun Takahashi, Kyoto, JP;
Daisuke Doi, Kyoto, JP;
Bumpei Samata, Kyoto, JP;
Kiyotoshi Sekiguchi, Suita, JP;
Yuichi Ono, Kobe, JP;
Kyoto University, Kyoto, JP;
Osaka University, Osaka, JP;
Eisai R&D Management Co., Ltd., Tokyo, JP;
Abstract
The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGFβ inhibitor, SHH signal-stimulating agent, FGF8, and GSK3β inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.