The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 18, 2022
Filed:
Oct. 28, 2016
Hoffmann-la Roche, Inc., Little Falls, NJ (US);
Detlef Eisenkraetzer, Iffeldorf, DE;
Christian Klinger, Penzberg, DE;
Katrin Greppmair, Penzberg, DE;
Claudia Schmidberger, Staig, DE;
Hoffmann-La Roche, Inc., Little Falls, NJ (US);
Abstract
The invention relates to a system () for monitoring deviations of a state of a cell culture in a bioreactor () from a reference state of a cell culture in a reference bioreactor (). The bioreactor comprises the same medium (M) as the reference bioreactor. The system comprises: •—a storage medium () comprising: •a PACO-reference profile () indicative of a deviation of a CO2 off gas rate (ACO-M-) measured in the reference bioreactor from a predicted CO2 off gas rate (ACO) of the reference bioreactor; •a data object comprising a medium-specific relation () between the pH value of the medium (M) and a respective fraction of CO2 gas in a gas volume when said medium is in pH-CO2 equilibrium state with said gas volume and lacks the cell culture; •—an interface () for receiving () a current CO2 off gas rate (ACO-M-ti, ACO-M-i) and a current pH value (pH) of the medium of the bioreactor (); •—a comparison unit () configured for computing (): •a PACO value (PACOPACO) the PACO-value being indicative of a deviation of a CO2 off gas rate (ACO-M-ti, ACO-M-) measured in the bioreactor from a predicted CO2 off gas rate (ACO-ti, ACOxp-i). a difference between the computed PACO value (PACO, PACO) and a respective reference PACO value (PACO) in the PACO-reference profile ().