The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 18, 2022
Filed:
Oct. 06, 2015
Applicant:
National University Corporation Tokyo Medical and Dental University, Tokyo, JP;
Inventors:
Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01K 67/027 (2006.01); C12N 15/873 (2010.01); C12N 15/11 (2006.01); C12N 9/22 (2006.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
A01K 67/0278 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/111 (2013.01); C12N 15/873 (2013.01); C12N 15/907 (2013.01); A01K 2217/072 (2013.01); A01K 2227/105 (2013.01); A01K 2267/01 (2013.01); C12N 2310/20 (2017.05); C12N 2330/51 (2013.01);
Abstract
An object of the present invention is to provide a method of conveniently producing a genetically modified non-human mammal with high efficiency using a CRISPR-Cas9 system and particularly a production method whereby gene knock-in can be achieved with high efficiency regardless of the gene size. The method of producing a genetically modified non-human mammal comprises introducing a Cas9 protein, a crRNA fragment comprising a nucleotide sequence complementary to a target DNA region, and a tracrRNA fragment into a non-human mammalian oocyte to genetically modify the target DNA.