The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 11, 2022
Filed:
Oct. 23, 2018
Applicant:
Shanghaitech University, Pudong New Area, CN;
Inventors:
Assignee:
ShanghaiTech University, Shanghai, CN;
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); C12N 9/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6845 (2013.01); C12N 9/93 (2013.01); C07K 2319/03 (2013.01); C07K 2319/70 (2013.01); G01N 2333/9015 (2013.01);
Abstract
Compositions and methods for detecting molecule-molecule interactions are provided. The methods employ a prokaryotic ubiquitin-like protein (Pup) and a Pup ligase that is coupled to one of the molecules. When the Pup ligase is brought to proximity to the other molecule by virtue of the molecule-molecule interaction, the Pup ligase can conjugate the Pup to a lysine residue on the other molecule. As such conjugation can be easily detected, this method allows easy identification of the molecule-molecule interaction.