The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 11, 2022

Filed:

Feb. 08, 2019
Applicant:

Immatics Us, Inc., Houston, TX (US);

Inventors:

Mamta Kalra, Houston, TX (US);

Zoe Coughlin, Houston, TX (US);

Amir Alpert, Houston, TX (US);

Steffen Walter, Houston, TX (US);

Ali Mohamed, Houston, TX (US);

Agathe Bourgogne, Houston, TX (US);

Assignee:

Immatics US, Inc., Houston, TX (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/17 (2015.01); C12N 5/0783 (2010.01); C07K 16/28 (2006.01); C12N 15/86 (2006.01); A61P 31/00 (2006.01); A61P 35/00 (2006.01);
U.S. Cl.
CPC ...
A61K 35/17 (2013.01); A61P 31/00 (2018.01); A61P 35/00 (2018.01); C07K 16/2809 (2013.01); C07K 16/2818 (2013.01); C12N 5/0636 (2013.01); C12N 5/0637 (2013.01); C12N 5/0638 (2013.01); C12N 15/86 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/2307 (2013.01); C12N 2501/2312 (2013.01); C12N 2501/2315 (2013.01); C12N 2501/2321 (2013.01); C12N 2501/51 (2013.01); C12N 2501/515 (2013.01); C12N 2510/00 (2013.01);
Abstract

The disclosure relates to methods of manufacturing T cells for adoptive immunotherapy. The disclosure further provides for methods of genetically transducing T cells, methods of using T cells, and T cell populations thereof. In an aspect, the disclosure provides for methods of thawing frozen peripheral blood mononuclear cells (PBMC), resting the thawed PBMC, activating the T cell in the cultured PBMC with an anti-CD3 antibody and an anti-CD28 antibody immobilized on a solid phase, transducing the activated T cell with a viral vector, expanding the transduced T cell, and obtaining expanded T cells.


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