The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 04, 2022
Filed:
Sep. 30, 2020
Xi'an Jiaotong University, Xi'an, CN;
Liyun Kong, Xi'an, CN;
Jiaqi Wang, Xi'an, CN;
Yan Liu, Xi'an, CN;
Min Wu, Xi'an, CN;
Lijuan Shi, Xi'an, CN;
Rui Zhang, Xi'an, CN;
Menglu Wu, Xi'an, CN;
Guoli Li, Xi'an, CN;
Feifei Qi, Xi'an, CN;
Le Ma, Xi'an, CN;
Xi'an Jiaotong University, Xi'an, CN;
Abstract
A method for simultaneously determining fat-soluble vitamins and carotenoids in serum, and belonging to the technical field of analytical chemistry, includes an ionic liquid (IL) or a binary mixed solvent composed of an IL and another solvent is adopted as an extractant; the biological samples are pre-treated by liquid-liquid extraction (LLE) and then detected by high-performance liquid chromatography (HPLC); retinyl acetate and trans-β-apo-8'-carotenal are adopted as the internal standards for fat-soluble vitamins and carotenoids, respectively; and the internal standard method is adopted to establish standard curves for quantification based on the retention time and the UV-Vis absorption spectrum. Compared with the existing methods, in the disclosure, the pretreatment process is simple and easy to be conducted, the sample can be prepared in a short time, and the toxic and harmful organic solvent is used at a reduced amount.