The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 04, 2022
Filed:
May. 04, 2018
Universite Paris Descartes, Paris, FR;
Institut National DE LA Sante ET DE LA Recherche Medicale, Paris, FR;
Assistance Publique-hopitaux DE Paris, Paris, FR;
Universite Paris Diderot, Paris, FR;
Universite Paris Nord, Villetaneuse, FR;
Universite DE Rouen, Mont-Saint-Aignan, FR;
Chu DE Rouen, Rouen, FR;
Adeline Melard, Morangis, FR;
Mélanie Bertine, Bretigny sur Orge, FR;
Véronique Avettand Fenoel, Paris, FR;
Florence Damond, Saint Mande, FR;
Christine Rouzioux, Paris, FR;
Diane Descamps, Paris, FR;
Marie Gueudin, Rouen, FR;
Jean-Christophe Plantier, Bois-Guillaume, FR;
UNIVERSITÉ PARIS CITÉ, Paris, FR;
ASSISTANCE PUBLIQUE-HOPITAUX DE PARIS, Paris, FR;
CHU DE ROUEN, Rouen, FR;
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE, Paris, FR;
UNIVERSITE DE ROUEN, Mont-Saint-Aignan, FR;
UNIVERSITÉ PARIS NORD, Villetaneuse, FR;
Abstract
The present invention relates to a method for detecting or quantifying deoxyribonucleic acid (DNA) of human immunodeficiency virus 2 (HIV-2) in a sample containing DNA comprising: a) performing a real-time polymerase chain reaction (PCR) on the sample, or a fraction thereof comprising DNA, with at least two sets of primers and probe each respectively comprising two primers and a labeled probe for the detection or quantification of HIV-2 DNA, at least one of the sets is selected from the group consisting of: a set comprising a primer comprising or consisting of a sequence SEQ ID NO: 1 or a sequence having al least 90% identity to SEQ ID NO: 1, a primer comprising or consisting of a sequence SEQ ID NO: 2 or a sequence having 90% identity to SEQ ID NO: 2 or the complement of these sequences, and a labeled probe comprising or consisting of a sequence SEQ ID NO: 3, or a sequence having at least 90% identify to SEQ ID NO: 3 or the complement of these sequences, and a set comprising a primer comprising or consisting of a sequence SEQ ID NO: 4 or a sequence having at least 90% identity to SEQ ID NO: 4, a primer comprising or consisting of a sequence SEQ ID NO: 5 or a sequence having 90% identity to SEQ ID NO: 5 or the complement of these sequences, and a labeled probe comprising or consisting of a sequence SEQ ID NO: 6 or a sequence having at least 90% identity to SEQ ID NO: 6 or the complement of these sequences, and b) determining therefrom the presence or absence and/or the quantity of HIV-2 DNA in the biological sample.