The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 04, 2022
Filed:
Jun. 29, 2018
Applicant:
University of Maryland, College Park, Waldorf, MD (US);
Inventors:
Lai-Xi Wang, Ellicott City, MD (US);
Xin Tong, College Park, MD (US);
Tiezheng Li, Columbia, MD (US);
Assignee:
University of Maryland, College Park, College Park, MD (US);
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/18 (2006.01); C07K 1/04 (2006.01); C07K 1/36 (2006.01); C07K 1/02 (2006.01); C07K 1/107 (2006.01); A61K 47/61 (2017.01); C12N 1/20 (2006.01); A61K 47/54 (2017.01); A61K 47/68 (2017.01); C07K 16/28 (2006.01); C07K 16/32 (2006.01); C12N 9/24 (2006.01); C12P 21/00 (2006.01); C12R 1/46 (2006.01); A61K 9/00 (2006.01);
U.S. Cl.
CPC ...
C07K 16/18 (2013.01); A61K 47/549 (2017.08); A61K 47/61 (2017.08); A61K 47/6803 (2017.08); C07K 1/02 (2013.01); C07K 1/045 (2013.01); C07K 1/1077 (2013.01); C07K 1/36 (2013.01); C07K 16/2887 (2013.01); C07K 16/32 (2013.01); C12N 1/205 (2021.05); C12N 9/2402 (2013.01); C12P 21/005 (2013.01); A61K 9/0019 (2013.01); C07K 2317/10 (2013.01); C07K 2317/24 (2013.01); C07K 2317/41 (2013.01); C12R 2001/46 (2021.05); C12Y 302/01096 (2013.01);
Abstract
The present invention provides for a one-pot enzymatic approach which does not require removal of the enzyme and purification of the intermediate after deglycosylation step, and the Endo-S treatment is able to do both deglycosylation and transglycosylation. The one-pot strategy of the present invention enables chemoenzymatic synthesis of an azido-tagged N-glycoform of monocloncal antibodies which could be further modified through orthogonal chemical ligation for various applications.