The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 04, 2022

Filed:

Oct. 13, 2021
Applicant:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Inventors:

Andrew Tustian, Millwood, NY (US);

Ankit Vartak, Elmsford, NY (US);

Thomas Daly, New City, NY (US);

Erica Pyles, New City, NY (US);

Nisha Palackal, White Plains, NY (US);

Shunhai Wang, Scarsdale, NY (US);

Ning Li, New Canaan, CT (US);

Assignee:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/36 (2006.01); C07K 14/71 (2006.01); C12P 21/06 (2006.01); C07K 1/113 (2006.01); C07K 1/16 (2006.01); C12N 5/00 (2006.01); C07K 1/18 (2006.01); C07K 1/22 (2006.01); C12P 21/02 (2006.01); A61K 38/18 (2006.01); C07K 16/28 (2006.01); C12N 15/66 (2006.01); C07K 16/22 (2006.01); G01N 30/80 (2006.01); G01N 30/02 (2006.01);
U.S. Cl.
CPC ...
C07K 14/71 (2013.01); A61K 38/1866 (2013.01); C07K 1/113 (2013.01); C07K 1/16 (2013.01); C07K 1/18 (2013.01); C07K 1/22 (2013.01); C07K 1/36 (2013.01); C07K 16/22 (2013.01); C07K 16/283 (2013.01); C12N 5/0018 (2013.01); C12N 5/0031 (2013.01); C12N 15/66 (2013.01); C12P 21/02 (2013.01); C12P 21/06 (2013.01); G01N 30/80 (2013.01); C07K 2317/622 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01); C12N 2800/10 (2013.01); G01N 2030/027 (2013.01);
Abstract

The present disclosure pertains to compositions comprising aflibercept and methods for producing such compositions in chemically defined media and using anion exchange chromatography to produce aflibercept with a specific glycosylation profile. In particular, these methods produce aflibercept with a glycosylation profile with specific ranges of % total fucosylated glycans, total sialylated glycans, mannose-5, and galactosylated glycans.


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