The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 04, 2022

Filed:

Nov. 08, 2017
Applicant:

The United States of America, As Represented BY the Secretary, Department of Health and Human Services, Bethesda, MD (US);

Inventors:

Kapil Bharti, Potomac, MS (US);

Min Jae Song, Olney, MD (US);

Russell Louis Quinn, Washington, DC (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61L 27/38 (2006.01); A61L 27/18 (2006.01); A61L 27/24 (2006.01); A61L 27/52 (2006.01); C12N 5/079 (2010.01); C12N 5/077 (2010.01); C12N 5/071 (2010.01); A61L 27/58 (2006.01); A61L 27/56 (2006.01);
U.S. Cl.
CPC ...
A61L 27/3813 (2013.01); A61L 27/18 (2013.01); A61L 27/24 (2013.01); A61L 27/3808 (2013.01); A61L 27/3891 (2013.01); A61L 27/52 (2013.01); C12N 5/069 (2013.01); C12N 5/0621 (2013.01); C12N 5/0656 (2013.01); A61L 27/56 (2013.01); A61L 27/58 (2013.01); A61L 2430/16 (2013.01); C12N 2500/38 (2013.01); C12N 2501/105 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2501/165 (2013.01); C12N 2501/17 (2013.01); C12N 2501/39 (2013.01); C12N 2501/90 (2013.01); C12N 2533/40 (2013.01); C12N 2533/50 (2013.01); C12N 2533/54 (2013.01);
Abstract

Methods are disclosed for fabricating a three-dimensional engineered blood retinal barrier (BRB) comprising a choroid and retinal pigment epithelial cells. The methods include the use of bioprinting. Also disclosed is a three-dimensional engineered BRB, and its use. Methods are also disclosed for using the three-dimensional engineered BRB, such as for the treatment of retinal degeneration in a subject or screening. A three-dimensional printing insert that is adapted for bioprinting on a culture substrate sheet that is securely retained within and exposed through a printing frame is also disclosed.


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