The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 27, 2022

Filed:

Jul. 28, 2016
Applicants:

The Johns Hopkins University, Baltimore, MD (US);

Duke University, Durham, NC (US);

Kennedy Krieger Institute, Inc., Baltimore, MD (US);

Inventors:

Anne Comi, Baltimore, MD (US);

Jonathan Pevsner, Baltimore, MD (US);

Zhenhua Huang, Ellicott City, MD (US);

Doug Marchuk, Chapel Hill, NC (US);

Assignees:

The Johns Hopkins University, Baltimore, MD (US);

Duke University, Durham, NC (US);

Kennedy Krieger Institute, Inc., Baltimore, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6883 (2018.01); C12Q 1/6886 (2018.01); A61K 31/7076 (2006.01); C12Q 1/68 (2018.01); A61P 17/00 (2006.01); A61P 35/00 (2006.01);
U.S. Cl.
CPC ...
A61K 31/7076 (2013.01); A61P 17/00 (2018.01); A61P 35/00 (2018.01); C12Q 1/68 (2013.01); C12Q 1/6883 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/142 (2013.01); C12Q 2600/156 (2013.01);
Abstract

In one aspect, the present invention features a method of inhibiting proliferation and/or reducing survival of a cell comprising a GNAQ polynucleotide or polypeptide having a R183Q or Q209L mutation, comprising contacting the cell with puromycin or a puromycin analog, thereby inhibiting proliferation and/or reducing survival of the cell. In another aspect, a method of treating a vascular malformation or related condition in a subject, comprising administering to the subject an effective amount of puromycin or a puromycin analog is featured. In another aspect, the present invention features a method of identifying a candidate agent that modulates a GNAQ R183Q or Q209L mutation-associated disease, comprising contacting a cell comprising a GNAQ polynucleotide or polypeptide having a R183Q or Q209L mutation with puromycin and a candidate agent and comparing viability of the contacted cell with a reference level of viability, wherein an alteration in viability indicates that the candidate agent modulates the GNAQ R183Q or Q209L mutation-associated disease.


Find Patent Forward Citations

Loading…