The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 20, 2022

Filed:

Feb. 23, 2018
Applicant:

Universitetet I Tromsø—norges Arktiske Universitet, Tromsø, NO;

Inventors:

Marcin Pierechod, Kvaløysletta, NO;

Nils Peder Willassen, Tomasjord, NO;

Ulli Rothweiler, Tomasjord, NO;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6806 (2018.01); C07K 14/195 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C07K 14/195 (2013.01); C12Q 2522/101 (2013.01); C12Q 2525/117 (2013.01); C12Q 2527/125 (2013.01); C12Q 2527/137 (2013.01);
Abstract

The present invention relates to the use of a single-strand DNA binding protein (SSB) which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions, to dehybridize a DNA molecule or to prevent hybridisation of a complementary ssDNA, wherein the SSB comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical to SEQ ID NO:1, or a functional fragment thereof, and wherein the DNA molecule or ssDNA is present in or exposed to a solution containing one or more of the following (i) at least 350 mM of sodium ions; (ii) at least 50 mM of potassium ions; (iii) at least 150 mM of magnesium ions; or (iv) at least 200 mM of calcium ions.


Find Patent Forward Citations

Loading…