The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 20, 2022

Filed:

Mar. 28, 2016
Applicant:

Hitachi, Ltd., Tokyo, JP;

Inventors:

Tomoyuki Sakai, Tokyo, JP;

Masataka Shirai, Tokyo, JP;

Assignee:

HITACHI, LTD., Tokyo, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12M 1/34 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502761 (2013.01); C12M 1/34 (2013.01); C12N 15/1013 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0893 (2013.01); B01L 2400/086 (2013.01);
Abstract

A system for capturing a biomolecule in a single cell includes: a two-dimensional array having single cell capture holes on one surface of a substrate, and biomolecule capture areas inside the substrate each comprising a biomolecule capture member for capturing a biomolecule extracted from individual cells respectively captured by the single cell capture holes; a flow channel for flowing a sample containing cells to be assayed, from a 1st direction parallel to the surface of the substrate; a structure on the surface of the substrate and opposed to the 1st direction on the downstream side of each of the single cell capture holes; a 1st application means applying a 1st flow, and a 1st control means; and a 2nd application means applying a 2nd flow orthogonal to the one surface of the substrate toward each biomolecule capture area from each corresponding single cell capture hole, and a 2nd control means.


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