The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 13, 2022

Filed:

Feb. 19, 2018
Applicants:

Mitsui Chemicals, Inc., Tokyo, JP;

National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, JP;

Savid Therapeutics Inc., Tokyo, JP;

Inventors:

Tsuneji Suzuki, Sodegaura, JP;

Yoshiyuki Totani, Sodegaura, JP;

Kosuke Mano, Sodegaura, JP;

Shinichi Banba, Sodegaura, JP;

Haruhiko Kamada, Ibaraki, JP;

Taisuke Nakayama, Ibaraki, JP;

Hiroki Akiba, Ibaraki, JP;

Kouhei Tsumoto, Ibaraki, JP;

Tsuyoshi Inoue, Ibaraki, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/58 (2006.01); G01N 33/68 (2006.01); C07K 16/28 (2006.01); C07K 14/36 (2006.01); C07D 495/04 (2006.01); C07D 519/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/58 (2013.01); C07D 495/04 (2013.01); C07D 519/00 (2013.01); C07K 14/36 (2013.01); C07K 16/2878 (2013.01); G01N 33/68 (2013.01);
Abstract

Finding a protein of a minute amount present on a cell membrane to provide a method for producing an antibody against the protein. Producing an antibody using a protein identified by an identification method including: a labeling step of using a labeling agent comprising at least one selected from bis-iminobiotin compounds and bis-biotin compounds to obtain cells having a labeled protein; a degradation step of preparing a degradation product for an immobilization treatment, the degradation product containing the labeled protein; an immobilization step of immobilizing the labeled protein contained in the degradation product for an immobilization treatment on a stationary phase via a streptavidin mutant; a cleavage step of releasing an analysis sample from the stationary phase on which the labeled protein is immobilized; and an analysis step of analyzing the analysis sample to identify the labeled protein.


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