The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 13, 2022

Filed:

Aug. 24, 2017
Applicant:

Thermo Fisher Scientific Oy, Vantaa, FI;

Inventors:

Mikko Koskinen, Vantaa, FI;

Leena Valmu, Vantaa, FI;

Joni Rannisto, Vantaa, FI;

Kelly Flook, Bedford, MA (US);

Roger Grist, Ninebanks, GB;

Anssi Rantakari, Vantaa, FI;

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/569 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56944 (2013.01); G01N 33/569 (2013.01); G01N 33/56911 (2013.01); G01N 33/56916 (2013.01); G01N 33/56938 (2013.01); G01N 33/56961 (2013.01); G01N 33/6848 (2013.01);
Abstract

The present invention includes a novel method and system for identification of microorganisms in samples that proteins and other biological material from non-microorganism sources (e.g., proteins of mammalian origin) that can interfere with identification of the microorganisms. The methods and systems described herein include use of a single-use chromatography medium to purify intact proteins prior to mass spectrometry analysis. The chromatography medium and the methods described herein can rapidly and efficiently remove of a substantial portion of interfering biological material (e.g., mammalian proteins) from a crude cell lysate while preserving high signal strength and removing enough of the interfering protein(s) to allow for identification of the microorganism(s) by mass spectrometry analysis.


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