The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 13, 2022

Filed:

Nov. 23, 2018
Applicant:

Konkuk University Industrial Cooperation Corporation, Seoul, KR;

Inventors:

Jung-Kul Lee, Seoul, KR;

Liaoyuan Zhang, Seoul, KR;

Jing-Lin Li, Seoul, KR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/88 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12P 7/18 (2006.01); C12P 7/26 (2006.01); C12P 7/16 (2006.01); C12N 9/04 (2006.01);
U.S. Cl.
CPC ...
C12N 9/88 (2013.01); C12N 9/0004 (2013.01); C12N 9/0006 (2013.01); C12N 9/93 (2013.01); C12P 7/16 (2013.01); C12P 7/18 (2013.01); C12P 7/26 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/01004 (2013.01); C12Y 106/03001 (2013.01); C12Y 402/01028 (2013.01);
Abstract

In a method for producing acetoin, butanediol, or butanol from ethanol according to the present invention, a cell-free catalysis method was used by designing an artificial synthetic pathway so that proteins of NOX, EtDH, FLS, BDH, and DDH and variant proteins thereof exhibit cascade catalytic activity as enzymes. Compared to existing fermentation methods using microorganisms, the production method according to the present invention does not require cell growth and has a short synthetic pathway, a fast reaction rate, high yield and productivity, adjustment of targeted reaction conditions is convenient, and butanol may be effectively produced. Moreover, same may be reused numerous times by fixing the proteins to nano-particles, and are also effective for producing acetoin, butanediol, or butanol, thus being economical. Therefore, the production method may be usefully adopted in the relevant industries requiring acetoin, butanediol, or butanol.


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