The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 13, 2022

Filed:

Jun. 25, 2020
Applicant:

Roswell Biotechnologies, Inc., San Diego, CA (US);

Inventors:

Chulmin Choi, San Diego, CA (US);

Sungho Jin, San Diego, CA (US);

Paul W. Mola, San Diego, CA (US);

Barry L. Merriman, San Diego, CA (US);

Assignee:

ROSWELL BIOTECHNOLOGIES, INC., San Diego, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/00 (2006.01); G01N 27/327 (2006.01); C23C 14/16 (2006.01); C23C 14/48 (2006.01); C23F 4/00 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502707 (2013.01); B01L 3/502715 (2013.01); C12Q 1/00 (2013.01); C12Q 1/6869 (2013.01); C23C 14/16 (2013.01); C23C 14/48 (2013.01); C23F 4/00 (2013.01); G01N 27/3278 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/0896 (2013.01);
Abstract

A method of manufacturing a device useable in DNA or genome sequencing comprises disposing pairs of electrodes on a substrate, the electrodes within each pair separated by a nanogap; depositing a resist layer over the electrodes; patterning the resist layer to create an exposed region on each electrode at or near each nanogap; roughening the electrode surface within each exposed region using various methods; and exposing the exposed regions to biomolecules, wherein one biomolecule bridges each nanogap of each electrode pair, with each end of each biomolecule bound to the electrodes at each exposed region.


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