The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 06, 2022

Filed:

Dec. 16, 2019
Applicant:

Waters Technologies Corporation, Milford, MA (US);

Inventors:

Joomi Ahn, Johnston, RI (US);

Moon Chul Jung, Waltham, MA (US);

Kevin D. Wyndham, Upton, MA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/37 (2006.01); C12N 11/06 (2006.01); C12N 11/14 (2006.01); C12M 1/40 (2006.01); C12M 1/34 (2006.01); C12Q 1/48 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/37 (2013.01); C12M 21/18 (2013.01); C12M 41/40 (2013.01); C12N 11/06 (2013.01); C12N 11/14 (2013.01); C12Q 1/48 (2013.01);
Abstract

An immobilized enzymatic reactor can include a wall defining a chamber having an inlet and an outlet; a solid stationary phase covalently linked to an enzyme and disposed within the chamber; and a pressure modulator in fluid communication with the chamber and adapted to support continuous flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet under a pressure between about 2,500 and 35,000 psi. In one example, the solid stationary phase includes inorganic/organic hybrid particles in an ultra performance liquid chromatography system, the enzyme is a protease, and the polymer analyte is a polypeptide. The immobilized enzymatic reactor can prepare an analyte for applications such as for hydrogen deuterium exchange mass spectrometry.


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