The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 06, 2022
Filed:
Dec. 07, 2016
Mgi Tech Co., Ltd., Guangdong, CN;
Longqi Liu, Guangdong, CN;
Chuanyu Liu, Guangdong, CN;
Liang Wu, Guangdong, CN;
Zhouchun Shang, Guangdong, CN;
Mengnan Cheng, Guangdong, CN;
Yue Yuan, Guangdong, CN;
Liqin Xu, Guangdong, CN;
Xin Liu, Guangdong, CN;
Xun Xu, Guangdong, CN;
MGI TECH CO., LTD., Guangdong, CN;
Abstract
Provided in the present invention is a method for constructing single cell sequencing libraries, comprising the following steps: a) lysing a single cell to obtain a single cell lysate; b) separating the nucleus and the cytoplasm in the single cell lysate obtained in step a) to obtain a nuclear solution and a total RNA solution; and c) constructing a chromatin DNA library with the nuclear solution obtained in step b) to obtain a chromatin-accessibility sequencing library of the single cell; and constructing a transcriptome library with the total RNA solution obtained in step b) to obtain a transcriptome sequencing library of the single cell.