The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 30, 2022
Filed:
Aug. 25, 2016
Applicant:
Duke University, Durham, NC (US);
Inventors:
Eric Josephs, Durham, NC (US);
Dewran Kocak, Durham, NC (US);
Piotr Marszalek, Durham, NC (US);
Charles A. Gersbach, Durham, NC (US);
Assignee:
Duke University, Durham, NC (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); G16B 25/00 (2019.01); G16B 15/10 (2019.01); G16B 30/10 (2019.01); G16B 15/30 (2019.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
C12N 15/11 (2013.01); C12N 15/111 (2013.01); C12N 15/907 (2013.01); G16B 15/10 (2019.02); G16B 15/30 (2019.02); G16B 25/00 (2019.02); G16B 30/10 (2019.02); C12N 2310/20 (2017.05);
Abstract
Disclosed herein are optimized guide RNAs (gRNAs) that have increased target binding specificity and reduced off-target binding. Further disclosed herein are methods of designing and using the optimized gRNAs.