The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 30, 2022

Filed:

Jul. 11, 2016
Applicant:

Novozymes A/s, Bagsvaerd, DK;

Inventors:

Tomoko Matsui, Chiba, JP;

Suzanne Clark, Youngsville, NC (US);

Aki Tomiki, Chiba, JP;

Assignee:

Novozymes A/S, Bagsvaerd, DK;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/44 (2006.01); C12P 7/06 (2006.01); C12P 19/02 (2006.01); C12P 19/14 (2006.01); C12C 5/00 (2006.01); C12C 7/04 (2006.01); C12N 9/34 (2006.01); C13K 1/06 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2457 (2013.01); C12C 5/00 (2013.01); C12C 5/004 (2013.01); C12C 7/04 (2013.01); C12N 9/2428 (2013.01); C12P 7/06 (2013.01); C12P 19/02 (2013.01); C12P 19/14 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01); C12Y 302/01041 (2013.01); C13K 1/06 (2013.01); C07K 2319/00 (2013.01); Y02E 50/10 (2013.01);
Abstract

The present invention relates to a variant pullulanase, wherein the pullulanase comprises at least the following combination of substitutions: N368G+N393A+Q431E+L432F+A492A,S+N610R+G624S+T631S+S632C, and optionally further comprises N222P+Q252A+Q256R; wherein the variant has pullulanase activity, and wherein the variants have at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3. Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.


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