The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 30, 2022

Filed:

Jun. 21, 2018
Applicant:

Cephalon, Inc., Frazer, PA (US);

Inventors:

Lu Wang, West Chester, PA (US);

Albert Kao, West Chester, PA (US);

Zhaoqing Zhang, West Chester, PA (US);

Mi Jin, West Chester, PA (US);

Tianyi Zhou, West Chester, PA (US);

Assignee:

Cephalon, Inc., Frazer, PA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 15/36 (2006.01); C07K 1/18 (2006.01); B01J 20/286 (2006.01); B01J 20/28 (2006.01); C07K 1/22 (2006.01); C07K 16/18 (2006.01); C12Q 1/686 (2018.01); C07K 16/00 (2006.01); C07K 16/28 (2006.01);
U.S. Cl.
CPC ...
B01J 20/286 (2013.01); B01D 15/362 (2013.01); B01J 20/28026 (2013.01); C07K 1/18 (2013.01); C07K 1/22 (2013.01); C07K 16/00 (2013.01); C07K 16/18 (2013.01); C07K 16/2875 (2013.01); C07K 16/2896 (2013.01); C12Q 1/686 (2013.01); C07K 2317/565 (2013.01);
Abstract

A wash buffer comprising a surfactant for use in affinity and cation exchange chromatography to purify proteins of interest from protein aggregates and to remove and/or inactivate viruses. When used during affinity or cation exchange chromatography for the purification of a protein of interest, such as an antibody, the wash buffer significantly improves viral clearance from the preparation, while also reducing the levels of host cell proteins and protein aggregates. Following affinity or cation exchange chromatography with the wash buffer, the protein of interest may be further purified using other chromatography and filtration operations.


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