The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 23, 2022

Filed:

Mar. 13, 2017
Applicant:

Isoplexis Corporation, Branford, CT (US);

Inventors:

Mong Sano Marma, Natick, MA (US);

Luisa Andruzzi, Concord, MA (US);

Ben McNally, Medford, MA (US);

Angela DeLucia, Cambridge, MA (US);

Dona Hevroni, Lexington, MA (US);

Elizabeth Carpenter, Hudson, MA (US);

Magnus Besev, Newton, MA (US);

Assignee:

IsoPlexis Corporation, Branford, CT (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6823 (2018.01); C07D 405/14 (2006.01); C07F 9/6561 (2006.01); C07D 405/04 (2006.01); C07D 491/22 (2006.01); C12Q 1/6874 (2018.01); C07H 19/14 (2006.01); C07H 19/10 (2006.01); C07D 519/00 (2006.01); C07F 9/6558 (2006.01); C07F 7/18 (2006.01); C09B 23/08 (2006.01); C09B 11/24 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6823 (2013.01); C07D 405/04 (2013.01); C07D 405/14 (2013.01); C07D 491/22 (2013.01); C07D 519/00 (2013.01); C07F 7/1804 (2013.01); C07F 9/6561 (2013.01); C07F 9/65583 (2013.01); C07H 19/10 (2013.01); C07H 19/14 (2013.01); C09B 11/24 (2013.01); C09B 23/083 (2013.01); C12Q 1/6874 (2013.01); Y02P 20/55 (2015.11);
Abstract

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3'-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.


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