The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 16, 2022
Filed:
Mar. 17, 2017
Applicant:
Delta Instruments B.v., Drachten, NL;
Inventors:
Pierre Laurent Emond, Amherst, NH (US);
Nancy Gail Perlmutter, Wellesley, MA (US);
James Willis Kreider, Newton, MA (US);
Assignee:
PERKINELMER HEALTH SCIENCES B.V., Groningen, NL;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/30 (2006.01); C12Q 1/04 (2006.01); A01B 5/02 (2006.01); C12M 1/34 (2006.01); B01L 3/00 (2006.01); C09B 11/28 (2006.01); C09B 23/04 (2006.01); C12Q 1/68 (2018.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
G01N 1/30 (2013.01); A01B 5/02 (2013.01); B01L 3/5023 (2013.01); B01L 3/50273 (2013.01); C09B 11/28 (2013.01); C09B 23/04 (2013.01); C12M 1/34 (2013.01); C12Q 1/04 (2013.01); C12Q 1/68 (2013.01); G01N 21/6486 (2013.01);
Abstract
The invention relates to a method for counting cells, such as bacteria and/or somatic cells in liquid samples, such as in dairy products, preferably raw milk. Disclosed is a method comprising a combination of steps that apply dimeric nucleic acid dyes that normally do not penetrate cells (=cell-impermeant dyes), which are rendered cell-permeant by using the right combination of pH, buffer and temperature.