The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 16, 2022

Filed:

Jan. 21, 2019
Applicant:

Wisconsin Alumni Research Foundation, Madison, WI (US);

Inventors:

John Ralph, Madison, WI (US);

Steven D Karlen, Verona, WI (US);

Fachuang Lu, Madison, WI (US);

Dharshana Padmakshan, Madison, WI (US);

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07C 69/00 (2006.01); C07C 69/73 (2006.01); C07C 69/007 (2006.01); C07C 69/16 (2006.01); C07C 69/732 (2006.01); C07C 69/734 (2006.01); C07C 69/84 (2006.01); C07C 69/86 (2006.01); C07C 69/92 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C07C 69/73 (2013.01); C07C 69/007 (2013.01); C07C 69/16 (2013.01); C07C 69/732 (2013.01); C07C 69/734 (2013.01); C07C 69/84 (2013.01); C07C 69/86 (2013.01); C07C 69/92 (2013.01); G01N 33/50 (2013.01); C07B 2200/05 (2013.01); G01N 2400/26 (2013.01); G01N 2458/15 (2013.01); G01N 2560/00 (2013.01);
Abstract

Methods of detecting and, optionally, determining a level of incorporation of monolignol ester conjugates into lignin. The methods include derivatizing lignin to acylate at least a portion of free phenolic and aliphatic hydroxyls and to halogenate at least a portion of benzylic alcohols present in the lignin to yield derivatized lignin, treating the derivatized lignin with a reducing agent to cleave at least a portion of the derivatized lignin to yield lignin cleavage products, acetylating at least a portion of free hydroxyl groups in the lignin cleavage products with a labeled acetylation agent to yield labeled lignin fragments, and detecting the labeled lignin fragments.


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