The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 09, 2022

Filed:

Aug. 13, 2020
Applicant:

Wisconsin Alumni Research Foundation, Madison, WI (US);

Inventors:

Melissa C. Skala, Middleton, WI (US);

Anthony Gitter, Madison, WI (US);

Zijie Wang, Madison, WI (US);

Alexandra J. Walsh, Madison, WI (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G06V 20/69 (2022.01); C12N 5/0783 (2010.01); G06K 9/62 (2022.01); G06V 10/44 (2022.01);
U.S. Cl.
CPC ...
G06V 20/698 (2022.01); C12N 5/0636 (2013.01); G06K 9/6257 (2013.01); G06K 9/6263 (2013.01); G06K 9/6271 (2013.01); G06V 10/454 (2022.01); G06V 20/695 (2022.01);
Abstract

Systems and methods for classifying and/or sorting T cells by activation state are disclosed. The system includes a cell classifying pathway, a single-cell autofluorescence image sensor, a processor, and a non-transitory computer-readable memory. The memory is accessible to the processor and has stored thereon a trained convolutional neural network and instructions. The instructions, when executed by the processor, cause the processor to: a) receive the autofluorescence intensity image; b) optionally pre-process the autofluorescence intensity image to produce an adjusted autofluorescence intensity image; c) input the autofluorescence intensity image or the adjusted autofluorescence intensity image into the trained convolutional neural network to produce an activation prediction for the T cell.


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