The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 09, 2022

Filed:

Jan. 25, 2022
Applicant:

Inscripta, Inc., Boulder, CO (US);

Inventors:

Phillip Belgrader, Pleasanton, CA (US);

Christian Siltanen, Boulder, CO (US);

William Watterson, Boulder, CO (US);

Burak Dura, Boulder, CO (US);

Bruce Chabansky, Boulder, CO (US);

David Stumbo, Boulder, CO (US);

Eric Smith, Boulder, CO (US);

Jorge Bernate, Boulder, CO (US);

Assignee:

Inscripta, Inc., Boulder, CO (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 15/63 (2006.01); C12N 15/85 (2006.01); C07H 21/04 (2006.01); C12N 15/10 (2006.01); C12M 1/34 (2006.01); C12M 1/26 (2006.01); C12N 15/113 (2010.01); C12N 15/86 (2006.01); C12M 3/00 (2006.01); C12M 3/04 (2006.01); C12M 1/00 (2006.01); C12N 5/074 (2010.01); C12N 15/11 (2006.01); C12N 15/88 (2006.01); C12N 15/90 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12M 1/32 (2006.01); C12M 3/06 (2006.01); C12N 9/22 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1082 (2013.01); B01L 3/502761 (2013.01); B01L 7/00 (2013.01); C12M 23/12 (2013.01); C12M 23/16 (2013.01); C12M 23/42 (2013.01); C12M 23/50 (2013.01); C12M 27/10 (2013.01); C12M 29/04 (2013.01); C12M 33/14 (2013.01); C12M 41/36 (2013.01); C12M 43/00 (2013.01); C12M 47/02 (2013.01); C12M 47/04 (2013.01); C12N 5/0696 (2013.01); C12N 15/1065 (2013.01); C12N 15/1068 (2013.01); C12N 15/11 (2013.01); C12N 15/113 (2013.01); C12N 15/86 (2013.01); C12N 15/88 (2013.01); C12N 15/907 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/123 (2013.01); B01L 2300/161 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0424 (2013.01); C12N 9/22 (2013.01); C12N 2310/20 (2017.05); C12N 2510/00 (2013.01); C12N 2740/10011 (2013.01); C12N 2740/15011 (2013.01); C12N 2750/14111 (2013.01); C12N 2800/80 (2013.01);
Abstract

This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells. The present compositions and methods entail viral delivery of an editing cassette to live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow, preferably using a fully-automated end-to-end instrument to process the cells without human intervention to enhance cell processing uniformity and to maintain the integrity of the cell culture.


Find Patent Forward Citations

Loading…