The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 26, 2022

Filed:

Jun. 21, 2018
Applicant:

Biofire Diagnostics, Llc, Salt Lake City, UT (US);

Inventors:

Benjamin William Galvin, Salt Lake City, UT (US);

Thomas Charles Robbins, Salt Lake City, UT (US);

Charles Benjamin Cox, Sandy, UT (US);

Assignee:

BIOFIRE DIAGNOSTICS, LLC, Salt Lake City, UT (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); C12Q 1/689 (2018.01); C12Q 1/70 (2006.01); G16B 30/00 (2019.01); G16B 40/10 (2019.01);
U.S. Cl.
CPC ...
G16B 40/10 (2019.02); C12Q 1/686 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/70 (2013.01); G16B 30/00 (2019.02);
Abstract

Methods, sample vessels, and instruments are provided for melting curve analysis. A sample is amplified by thermal cycling the sample well between at least an annealing temperature and a denaturation temperature through n cycles, where each cycle includes an in-cycle denaturation step. Fluorescent data is collected during the in-cycle denaturation step of n cycles where n is at least two. Then a composite melting curve is generated using the fluorescent data collected during the denaturation step of each of the n cycles, and the sample is called using the composite melting curve, where the call is a positive or a negative call.


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