The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 26, 2022
Filed:
Feb. 10, 2017
Max-delbrück-centrum Für Molekulare Medizin IN Der Helmholtz-gemeinschaft, Berlin, DE;
Genetic Information Research Institute, Cupertino, CA (US);
Tilmann Buerckstuemmer, Cambridge, GB;
Vladimir Vyacheslavovich Kapitonov, Mountain View, CA (US);
Ivana Grabundzija, Berlin, DE;
Zoltan Ivics, Berlin, DE;
MAX-DELBRÜCK-CENTRUM FÜR MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT, Berlin, DE;
GENETIC INFORMATION RESEARCH INSTITUTE, Cupertino, CA (US);
Abstract
The invention relates to a system and method for introducing DNA into cells. In particular, the invention relates to a method for introducing single or multiple copies of a DNA sequence or gene of interest into a cell comprising providing: a) a 'copy and paste' transposase; and b) a construct comprising a DNA sequence or gene of interest flanked by a 'copy and paste' transposon terminal sequence, such as an LTS or RTS. A novel “copy and paste” transposon of the Helitron family is described along with systems for using the corresponding transposase in methods for introducing DNA into cells, for example, to generate cell lines for use in protein production, cell and gene therapy or as reference standards.