The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 19, 2022

Filed:

Feb. 14, 2019
Applicant:

Korea Research Institute of Chemical Technology, Daejeon, KR;

Inventors:

SunHyun Park, Daejeon, KR;

Ki-Suk Kim, Daejeon, KR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/30 (2006.01); G01N 33/532 (2006.01); G01N 33/68 (2006.01); G01N 33/577 (2006.01);
U.S. Cl.
CPC ...
G01N 1/30 (2013.01); G01N 33/532 (2013.01); G01N 33/577 (2013.01); G01N 33/6854 (2013.01); G01N 33/6872 (2013.01); G01N 2001/302 (2013.01);
Abstract

The present invention relates to a composition for immunostaining cleared large tissues and a method for immunostaining cleared large biological tissues using the same. The composition for immunostaining cleared large tissues increases antibody permeability in cleared biological tissues, in particular, large biological tissues having a thickness of 3 mm or more, to allow antibodies to penetrate deep into thick tissues, thereby overcoming immunostaining problems associated with limited permeation of antibodies in existing methods, reduces the time required for immunostaining large tissues, thereby enabling simple and rapid three-dimensional bioimaging with high resolution for large biological tissues, and may be beneficially used, through structure images, for identifying the causes of various diseases, developing new treatment methods, and predicting the efficacy and toxicity of drugs. Furthermore, the composition for immunostaining cleared large tissues may be used in combination with various medical devices, and in particular, may be prepared as a kit and beneficially used as an in vitro diagnostic device.


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