The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 19, 2022
Filed:
Sep. 01, 2016
University of Massachusetts, Boston, MA (US);
University of Central Florida Research Foundation, Inc., Orlando, FL (US);
Thoru Pederson, Worcester, MA (US);
Hanhui Ma, Shrewsbury, MA (US);
Li-Chun Tu, Worcester, MA (US);
Ardalan Naseri, Orlando, FL (US);
Maximilaan Huisman, Worcester, MA (US);
Shaojie Zhang, Orlando, FL (US);
UNIVERSITY OF MASSACHUSETTS, Boston, MA (US);
UNIVERSITY OF CENTRAL FLORIDA FOUNDATION, INC., Orlando, FL (US);
Abstract
A C9orf72 DNA repeat expansion can be detected using a CRISPR Arrayed Repeat Detection System (CARDS). Based upon the compositions and methods supporting this platform primary cell cultures and/or blood cell smears can be tested under conventional clinical diagnostic laboratory conditions to diagnose genetically-based diseases having DNA repeat expansions, including but not limited to ALS. dCas9 constructs are also contemplated as having fluorescent proteins bound to any or all stem loop sequences, wherein detection of a plurality of dCas9 constructs having different colored fluorescent proteins can simultaneously detect at least six (6) different gene target loci.