The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 19, 2022

Filed:

Mar. 05, 2013
Applicant:

Agilent Technologies, Inc., Santa Clara, CA (US);

Inventors:

Michael Ruvolo, Santa Clara, CA (US);

Emily Marine Leproust, Santa Clara, CA (US);

Assignee:

Agilent Technologies, Inc., Santa Clara, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C07H 21/04 (2006.01); C12Q 1/6811 (2018.01); C12Q 1/6876 (2018.01); C12Q 1/6806 (2018.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6811 (2013.01); C12N 15/1068 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6876 (2013.01);
Abstract

Provided herein is a method for making a pool of probes by primer extension. In certain embodiments, the method comprises hybridizing a first population of oligonucleotides comprising a top strand sequence having the following formula V1-B-3' with a second population of oligonucleotides comprising a bottom strand sequence having the following formula V2′-B′-3′ to provide a population of duplexes. After hybridizing, the 3′ ends of the oligonucleotides in the duplexes are extended to produce a population of double stranded products comprising a top strand sequence having the following formula V1-B-V2, where V2 is complementary to V2′.


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