The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 19, 2022

Filed:

Jan. 26, 2015
Applicants:

Medicago Inc., Quebec, CA;

Universite Laval, Quebec, CA;

Inventors:

Marc-Andre D'Aoust, Quebec, CA;

Manon Couture, St-Augustin-de-Desmaures, CA;

Louis-Philippe Vezina, Neuville, CA;

Dominique Michaud, Quebec, CA;

Philippe Varennes-Jutras, Montreal, CA;

Frank Sainsbury, St. Lucia, AU;

Assignees:

MEDICAGO INC., Quebec, CA;

UNIVERSITE LAVAL, Quebec, CA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8258 (2013.01); C12N 15/8221 (2013.01); C12N 15/8257 (2013.01); C12N 2760/16122 (2013.01); C12N 2760/16151 (2013.01); C12N 2800/40 (2013.01); C12N 2820/60 (2013.01); C12N 2830/60 (2013.01);
Abstract

A method of increasing the yield, stability, or both of an acid sensitive protein in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, or portion of the plant. The first nucleic acid comprises a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding the acid sensitive protein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a channel protein, for example but not limited to a proton channel protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby increasing the yield of the acid sensitive protein when compared to the yield of the acid sensitive protein produced in the plant or portion of the plant produced under the same conditions, and in the absence of the proton channel protein.


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