The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 12, 2022

Filed:

Sep. 30, 2019
Applicant:

Government of the United States, As Represented BY the Secretary of the Air Force, Wright-Patterson AFB, OH (US);

Inventors:

Joseph M Slocik, Dayton, OH (US);

Rajesh R. Naik, Centerville, OH (US);

Patrick B Dennis, Cincinnati, OH (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 27/447 (2006.01); B01L 3/00 (2006.01); G01N 27/327 (2006.01); C07K 1/10 (2006.01); C12N 11/18 (2006.01); C07K 1/113 (2006.01); C12P 21/00 (2006.01); C07K 14/415 (2006.01); A61K 47/54 (2017.01); A01D 45/28 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44791 (2013.01); B01L 3/502761 (2013.01); G01N 27/3278 (2013.01); A01D 45/28 (2013.01); A61K 47/541 (2017.08); B01L 2200/0663 (2013.01); C07K 1/10 (2013.01); C07K 1/113 (2013.01); C07K 14/415 (2013.01); C12N 11/18 (2013.01); C12P 21/00 (2013.01); C12Y 110/03009 (2013.01);
Abstract

A method of forming a stable protein complex comprising: providing aqueous protein complexes, wherein the protein complexes are one or more of photosystem I complex from spinach, photosystem II complex from spinach, chlorophyll antennae, thylakoids, bacteriochlorophylls, chlorosomes, and photosystems from green algae, cyanobacteria, and plants; cationizing the aqueous protein complexes by the addition of stoichiometric amounts of a crosslinker in the presence of a coupling reagent; titrating the cationized protein complexes with a counter anionic polymer until the protein cation/anion pair solution becomes negative by zeta potential measurement, to create at least one antibody cation/anion pair in aqueous solution. The protein complexes cation/anion pair solution may be lyophilized to remove all of the water, forming a lyophilized solid. The lyophilized solid may be heated until a protein complex ionic liquid is generated. The cationized protein complexes may be purified from excess coupling reagents by dialysis in water.


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