The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 05, 2022

Filed:

Oct. 20, 2017
Applicants:

Vito NV, Mol, BE;

Universiteit Antwerpen, Antwerp, BE;

Inventors:

Dirk Valkenborg, Hasselt, BE;

Jef Hooyberghs, Mol, BE;

Frank Sobott, Leeds, GB;

Kris Laukens, Wilrijk, BE;

Frederik Lermyte, Hoevenen, BE;

Assignees:

VITO NV, Mol, BE;

Universiteit Antwerpen, Antwerp, BE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); G16Z 99/00 (2019.01); H01J 49/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6848 (2013.01); G16Z 99/00 (2019.02); H01J 49/0036 (2013.01); G01N 2560/00 (2013.01);
Abstract

The present invention provides a method for the determination of the monoisotopic mass of a macromolecule from a mass Mspectrometry spectrum of said macromolecule based on the experimentally determined most abundant mass, with accuracy in the low parts-per-million (ppm) range. The method uses a simple, double-linear model for predicting the monoisotopic mass based on the experimentally determined most abundant mass, comprising the steps of (a) deriving the most abundant mass MMostAb from the spectrum; and (b) calculating the monoisotopic mass Mfrom the most abundant mass M, using M=a+βM+ε; wherein β is a scalar slope obtainable by fitting the slope of monoisotopic mass versus most abundant mass for a plurality of macromolecules from a macromolecule database; and ε is a scalar residue of the form ε=ε+s, εbeing an integer, and εbeing a sawtooth function of M.


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