The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 28, 2022

Filed:

Feb. 27, 2019
Applicant:

Shimadzu Corporation, Kyoto, JP;

Inventor:

Mitsuhiro Kinoshita, Osaka, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/58 (2006.01); G01N 27/447 (2006.01); G16C 20/20 (2019.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/582 (2013.01); G01N 27/44791 (2013.01); G16C 20/20 (2019.02); B01L 3/5027 (2013.01); G01N 2550/00 (2013.01); G01N 2570/00 (2013.01);
Abstract

A sample, which is a mixture of glycans, is fluorescently labeled (S). The sample is subsequently separated by microchip electrophoresis under a buffer solution with no lectin added as well as under multiple kinds of buffer solutions with different lectins respectively added, and the separated components are fluorescently detected (S). A high-concentration gel which can produce a molecular-sieving effect is used as the buffer solution. Multiple electropherograms are created from the detection results (S). A glycan having a lectin specifically attached is delayed during its migration in the buffer solution, so that a peak corresponding to this glycan will effectively disappear. Accordingly, based on the kinds of lectins and the presence/absence of a peak on each of the electropherograms, the structure of each glycan in the sample is estimated and the glycan is identified (S).


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