The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 28, 2022
Filed:
Jun. 11, 2018
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventor:
Andrew F. Gardner, Manchester, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6855 (2018.01); C12N 9/22 (2006.01); C12N 9/00 (2006.01); C12Q 1/6874 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6855 (2013.01); C12N 9/22 (2013.01); C12N 9/93 (2013.01); C12Q 1/6874 (2013.01);
Abstract
Provided herein, among other things, are various in vitro methods that involve cleaving dsDNA molecules that comprise a mismatched nucleotide using EndoMS. In some embodiments, the method may comprise ligating a T-tailed double-stranded adapter to A-tailed double-stranded fragments of nucleic acid to produce ligation products that comprise adapter-ligated fragments and double-stranded adapter dimers that comprise a T:T mismatch at the ligation junction and cleaving both strands of the adapter dimers using EndoMS.