The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 28, 2022
Filed:
Jun. 08, 2017
Applicant:
Takara Bio Usa, Inc., San Jose, CA (US);
Inventors:
Thomas D. Schaal, San Jose, CA (US);
Jude Menlo Dunne, San Jose, CA (US);
Maithreyan Srinivasan, San Jose, CA (US);
Alain Mir, San Jose, CA (US);
Assignee:
Takara Bio USA, Inc., San Jose, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6853 (2018.01); C12P 19/34 (2006.01); C12Q 1/6844 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); C12P 19/34 (2013.01); C12Q 1/6844 (2013.01);
Abstract
The present disclosure provides methods, compositions, and systems employing blocked primers. Aspects of the disclosure include providing a blocked primer reaction mixture that includes a blocked primer and a template nucleic acid component from a single cell; unblocking the blocked primer to produce an active primer reaction mixture and subjecting the activated primer reaction mixture to primer extension conditions, such as nucleic acid amplification conditions.