The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 28, 2022
Filed:
Jan. 15, 2020
Applicant:
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Inventors:
Katherine Lazaruk, San Jose, CA (US);
Eleen Shum, San Jose, CA (US);
Christina Chang, San Jose, CA (US);
Assignee:
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6851 (2018.01); C12Q 1/6876 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12Q 1/6851 (2013.01); C12Q 1/6876 (2013.01); C12Q 2531/113 (2013.01); C12Q 2563/185 (2013.01);
Abstract
Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.