logo

For the Inventor, By the Inventor

The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11371046 B1

PDFFull Text
Date of Patent:
Jun. 28, 2022

Filed:

Sep. 20, 2016

Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors

Applicant:

Genomatica, Inc., San Diego, CA (US);

Inventors:

Mark J. Burk, San Diego, CA (US);

Stephen J. Van Dien, San Diego, CA (US);

Anthony P. Burgard, Bellefonte, PA (US);

Wei Niu, Lincoln, NE (US);

Assignee:

Genomatica, Inc., San Diego, CA (US);

Attorney:

Greenberg Traurig, LLP

Primary Examiner:

Channing S Mahatan

Int. Cl.
CPC ...
C12N 15/52 (2006.01); C12P 7/18 (2006.01); C12P 7/42 (2006.01); C12N 9/04 (2006.01); C12N 9/02 (2006.01); C12N 9/88 (2006.01); C12N 9/00 (2006.01); C12P 7/52 (2006.01); C12P 17/04 (2006.01); B01D 3/00 (2006.01); C12N 15/70 (2006.01); C12N 15/81 (2006.01);
U.S. Cl.
CPC ...
C12N 15/52 (2013.01); B01D 3/002 (2013.01); C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/88 (2013.01); C12N 9/93 (2013.01); C12N 15/70 (2013.01); C12N 15/81 (2013.01); C12P 7/18 (2013.01); C12P 7/42 (2013.01); C12P 7/52 (2013.01); C12P 17/04 (2013.01); C12Y 101/01061 (2013.01); C12Y 102/01076 (2013.01); C12Y 401/01071 (2013.01); C12Y 602/01004 (2013.01); Y02P 20/52 (2015.11);
Abstract

The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

Find Patent Forward Citations

Loading…