The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 28, 2022
Filed:
May. 08, 2017
Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, CN;
Min Zhou, Zhejiang, CN;
Xing Jiang, Zhejiang, CN;
Shuang Han, Zhejiang, CN;
Yingying He, Zhejiang, CN;
Linghui Zheng, Zhejiang, CN;
Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, CN;
Abstract
The present invention relates to a DoxA protein mutant having an amino acid sequence set forth in SEQ ID NO: 16, and coding gene thereof. The protein mutant or the coding gene thereof can be used for producing epirubicin. The present invention further relates to acapable of efficiently expressing epirubicin, which is constructed by replacing the dnmV gene of a startingin situ with the avrE gene and mutating the doxA gene of the startinginto a gene encoding the protein set forth in SEQ ID NO: 16. The fermentation broth of thishas an epirubicin potency of up to 102.0 μg/ml.