The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 28, 2022

Filed:

Aug. 16, 2019
Applicant:

Genzyme Corporation, Cambridge, MA (US);

Inventors:

Rohan Patil, Bridgewater, NJ (US);

Chad Varner, Bridgewater, NJ (US);

Assignee:

Genzyme Corporation, Cambridge, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61L 2/08 (2006.01); B01J 20/26 (2006.01); B01J 20/28 (2006.01); B01J 20/286 (2006.01); B01J 20/30 (2006.01); B01J 41/04 (2017.01); B01J 49/60 (2017.01); B01J 47/02 (2017.01); B01J 47/014 (2017.01); C07K 1/18 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); B01J 41/12 (2017.01);
U.S. Cl.
CPC ...
A61L 2/081 (2013.01); B01D 15/363 (2013.01); B01D 15/3809 (2013.01); B01J 20/26 (2013.01); B01J 20/286 (2013.01); B01J 20/28052 (2013.01); B01J 20/3071 (2013.01); B01J 41/04 (2013.01); B01J 41/12 (2013.01); B01J 47/014 (2017.01); B01J 47/02 (2013.01); B01J 49/60 (2017.01); C07K 1/18 (2013.01); B01J 2220/52 (2013.01);
Abstract

Provided herein are methods of reducing bioburden of a chromatography resin that include exposing a container including a composition including (i) a chromatography resin and (ii) a liquid including at least on alcohol to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one alcohol are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and a liquid including at least one alcohol, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified recombinant protein.


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